Abstract: Under the background of rapid development of innovation and entrepreneurship,this paper focused on the problem that turnover rate of New Generation Knowledge Workers in Internet Venture Companies was extremely high,and systematically explored the factors affecting the turnover intention of new generation knowledge workers. According to the integration research perspective and large sample empirical study,the result showed that:(1)7 factors had significant effect on the turnover intention, including job characteristics matching,investment return,organizational adaptability,group interaction effect,achievement motivation satisfaction,personal competence and external environment pressure.(2)different factors had different effects on turnover intention. The external environment had a negative effect on turnover intention while the other factors had a positive effect on turnover intention. (3) Different factors had different significant effect on turnover intention:job characteristics matching,investment return,organizational adaptability and group interaction effectiveness had the strongest effect; job characteristics matching; personal competence as well as external environment pressure were relatively weaker.