Abstract: With the continuous deepening of ecological civilization construction, the Chinese government is promoting Green Total Factor Productivity(GTFP)by issuing environmental regulations, which is of great practical significance to promoting high-quality economic development. Based on the panel data of 261 cities in China from 2003 to 2016, this paper tried to measure the change of urban GTFP by using the EBM-Windows-Malmquist-Luenberger model, which has both radial and non-radial characteristics, and then analyzed the impact of environmental regulations on urban GTFP by using the generalized moment estimation and the quantile regression estimator for panel data with non-additive fixed effects. This research has mainly obtained three results. Firstly, the changes of urban GTFP in the eastern, central, western and northeastern regions of China from 2003 to 2016, are of an "inverted U" shape. And the change of pure technical efficiency is the main factor affecting urban GTFP. Secondly, there is no positive or negative linear relationship between environmental regulations and urban GTFP, but these is a significant "inverted U" relationship between them. Thirdly, the influence of environmental regulations on GTFP at different sub-points shows obvious heterogeneity. That is, at a lower sub-point, environmental regulations have a greater impact on urban GTFP, and others do the opposite.